How to separate smooth muscle cells from fibroblasts in mouse prostate explant?

14 August 2018 0 8K Report

I was wondering if anyone had suggestions regarding a reliable protocol to get pure smooth muscle cultures, free from fibroblasts and epithelial cells, from prostate explants from mouse.

It seems that I will not be able to use magnetic microbeads because Miltenyi does not have beads to separate mouse fibroblasts.

Appreciate your responses.

Thank you.

Madhumita

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