Which mobile phase do you use in your TLC system? I separate neutral lipids from polar lipids with good results using silica TLC plates and n-hexane:diethyl ether:acetic acid 70:30:1 v/v/v as mobile phase. Be careful with the lipid amount you apply on the TLC plate, too much can lead to overlapping.

I used hexane : diethylether : acetic acid (80:20:2) for separation of neutral lipids i got very good results but what i want is to separete PL (PA, PC; PE and PA) for this separatio i used chloroform/acetic acid/methanol/water (70/25/5/2 v/v) as mobile phase

For PL class separation, I think that your mobile phase contains too much acetic acid. Try chloroform/methanol/acetic acid/water 100:45:5:2 by vol. It is better if you use also preparative TLC (20x20 cm plates) and this way, separation may be more effective.

Hi, in addition of the comments above, I think you can try to search in the Camag TLC data base for the suitable TLC system. I found 156 hits, for keyword: phospholipids, Best  reagards, gunawan

http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm?method=query

First Extracted for Pet.Ether and separated for LIPID AND THAN EXTRACTED FOR ETHER OR METHANOL