Is there some good Instrument for evaluate life's quality of patients with chronic disease?

I'm trying to conduct a research from this field. There's someone to help me?

09 December 2020 4,065 5 View

Optimal potassium to magnesium ratio in soils ?

Hi, After some research, I have found recommended soil potassium to magnesium ratios ranging from 0.25 to 0.7 in agricultural soils (ratios calculated from CEC concentrations). Which one do you...

26 July 2020 5,412 8 View

How to study the crystal structure of nano ferromagnetic materials? How to arrive at a suitable doping agent?

What are the books/articles to study the crystal structure of nanoferromagnetic materials?

31 March 2020 4,195 4 View

Hi, I'd like to stain mitochondria with TMRM in situ, anyone has a protocol?

I would like to see by confocal microscopy the activity of the mitochondria potential membrane using TMRM. The problem is that I can't find a way to stain at TMRM in situ. I can process the...

04 March 2020 5,033 2 View

Do you know good methods to describe the internal morphology of insect larvae?

I am looking for good methods to describe the internal morphology of hymenoptera larvae stages. If you know a related technique or protocol, I would appreciate if you indicate it.

22 February 2020 1,359 3 View

What is the maximum temperature the soil can reach in a ferruginous rock field?

I searched online but I could not find any answer to it. Does anyone ever measures it or knows a work on this topic with that info? Thanks :)

10 July 2019 1,415 3 View

What are the books/papers to learn about quantum confinement in 1D, 2D and 0D nanostructures?

What are the books/papers to learn about quantum confinement in 1D, 2D and 0D nanostructures? What are books/papers to know more about nanoelectronic devices?

07 January 2019 6,464 8 View

How to proceed with molecular dynamic simulations in nanoscale?

How to commence simulations in nanoscale? In which part of the code do we introduce the nano dimensions?

15 December 2018 9,749 6 View

Why do I obtain false positives in Datsenko strategy (red recombinase)?

Hi there! I am trying to obtain a knock-out using Datsenko strategy. I am working with pKD4 (as a template for PCR) and the pKD46 vector. Once I obtained the linear DNA (using primers with 50 bp...

09 December 2018 2,676 10 View

How to add a journal ?

23 October 2018 7,341 89 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View