I want to isolate the macrophage cell membrane and then coated it on nanoparticles. There are many protocols for the isolation of cell membrane vesicles, but I want to separate the macrophage cell membrane and next prepare cell membrane cracks. In addition, protocols suggest different hypotonic lysis buffers for macrophage cell membrane isolation (20 mM Tris-HCl, 10 mM KCl, 2 mM MgCl2, and 1 EDTA-free mini protease inhibitor tablet or 1 mmol L −1 NaHCO3, 0.2 mmol L −1 EDTA and 1 mmol L −1 PMSF or Tris-magnesium buffer (TM buffer, pH 7.4, 0.01 M Tris and 0.001 M MgCl2), which I don’t know the best one. After cell lysis, the protocols are followed by sucrose gradient or differential centrifugation, or sonication. I am worried about confirming the cell membrane after isolation and the best protocol for membrane isolation and saving them besides coating the nanoparticles with macrophage cell membrane cracks.

Any help is appreciated!