How to separate liposomes of different size?

Yuyan Liang @Yuyan-Liang-2

26 July 2021 1 10K Report

I want to separate 800nm and 50nm liposomes by centrifugation. What speed and time should I use?

Yuyan Liang

Sairam Dasika

Thanks for your answer, but I want to separate the liposomes by centrifugation. Is there any information?

Badges
Science topic

More Yuyan Liang's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Can we isolate microplastic from sludge sample without using vacuum filter..?

isolation of microplastic from sludge sample using centrifugation ..

23 July 2024 6,418 0 View

Any better protocol for entrapment efficiency of curcumin?

I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...

01 July 2024 7,007 1 View

Most accurate way to determine IC50 values by flow cytometry?

We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing...

23 June 2024 4,279 0 View

Alternative to Centrifuge software (Krumm, 1996)?

Dear colleagues, does anyone know of a suitable alternative for Centrifuge software (calculating settling time for grain size separation by centrifugation), preferably one running on Windows 10 or...

17 June 2024 7,085 0 View

P-nitrophenol acetate assay for esterase and media supernant colour interference. what can be done?

I am performing p nitrophenol acetate assay for esterase enzyme. My media contains a different concentration of peptone (0.5, 2, 5%) and after centrifugation the supernatant is slight yellow....

16 June 2024 4,381 0 View

Microwave assisted Solid Phase Peptide Synthesis resulting in problematic 'sticky' gel peptide product following centrifugation of diethyl ether?

I'm trying to synthesis a hydroscopic peptide using a microwave assisted solid phase peptide synthesiser. I'm using an already optimised cleavage process, the issue arises after precipitating the...

15 June 2024 2,728 2 View

How to visualize a cell palett of a small cell amount?

Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...

09 June 2024 6,311 0 View