How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Dflux and urdfil subroutine input in abaqus?

I am using coupled transient thermal electric step to simulate thermal and electric flux load on cfrp layered composite. DFLUX been used for surface heat flux and URDFIL for element deletion but...

25 December 2020 2,551 1 View

How stiffness matrix modification after hinge formation is done for pushover analysis?

Can anyone please explain this? How do I account for plastic hinges in my model and how do I modify the stiffness after plastic hinge is formed? Need a small example. Thank You

15 November 2020 1,967 3 View

How to model hysteresis property of concrete in abaqus?

I want to model rc element subjected to cyclic loading, How do I enter hysteresis data in the material model? I want to use Mander's 1988 model to get data for concrete. I dont know how to enter...

06 September 2020 7,651 3 View

Why the ZDOCK output file (.pdb) is not compatible for Chimera?

Why the final docked complex file output from ZDOCK is not compatible for Chimera?

24 April 2020 3,347 3 View

Got frequency of human recorded audio file in the range of 0-8000 Hz. Is it correct range?as per many sources human voice frequency is below 260 Hz?

I tried finding frequency by various tools such as audacity (got frequency 0-16000 Hz). Tried with Librosa(Python package) and got the frequency of human recorded audio files in the range of...

06 April 2020 7,607 6 View

Is there any software which take input as pdf(research papers) and generate the data from all the plots and graph inside it?(Similar to NLP)?

I am looking for software that will take input as a research paper (pdf format) and generate the data from plots and graph in it. as similar to NLP softwares.

04 February 2020 4,244 3 View

How to increase RNA yield and purity from PBMCs stored in RNA later at -80 C?

I have been using miRNA Isolation mini kit (qiagen 217004) for isolating RNA from PBMCs stored in RNA later at -80 C. The cell no. was 2 million cells. The RNA yields obtained are around 30-40...

15 October 2019 4,731 1 View

What is the criteria to select the range of normal stress in Direct shear test?

I did not not find the answer anywhere. Please help.

19 August 2019 9,950 3 View

How can the trigonometric expression be solved for particular variable in MATLAB?

When I try to solve the symbolic trigonometric expression, I am not getting desired results even results exist. The MATLAB code is written as follow: clear all clc syms nq lam alfa ai theta phi...

16 July 2019 1,380 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

The procedure compute?

02 March 2021 5,204 3 View