I am using frozen section to study the structure of intestinal tissues. Having made loads of frozen section slides recently, I unfortunatelly used wrong microscope slides which were not adhesion slides but normal ones. When I washed the slides with PBS, almost all the samples were washed away though I just washed them very gently. As I spent a whole week making those slides and maybe don't have time to redo the slicing procedure, I wonder if there's any remedial method to improve the adhesion of samples to slides and avoid losing too much samples? Thank you so much!
(p.s. To provide some background, the tissues were fixed in 4% pfa for about 3 hours and dehydrated in 30% sucrose for about 4 days, then froze in OCT at -80℃ and sliced at -20℃ in the frozen slicer)
You could try aceton, but I guess this can affect your samples as well, depending on what you plan to do next with them (as in active-LDH staining for example).
Hi! You can try prepping the slide with poly-L-lysine to change the polarity of the glass surface helping sample adhesion.
Hi, Jiahui Niu
Poly-L-lysine does not change cell morphology. This pretreatment on slides is common in Optical and Scanning Electron Microscopy Analyzes, for example.
I've never had a problem with cell morphological changes. In addition, it is a method that has been used for a long time.
I hope you can resolve this issue and succeed in your analysis.
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