In electronic microscope the we need high vacuum to attain high quality images.
How to safeguard our FESEM or TEM instrument from unknown sample releasing impurity while analysis?
Where as the unknown sample may release gaseous impurity even after drying and keeping in desiccator.
What are all the pre-analysis We need to do before insertion of unknown sample
Most of the (FESEM & TEM) are connected with the air compressor, it helps to collect the gaseous impurities released from the un known samples during analysis. So that, you should drain the air compressor periodically (Mostly one in a month is quite enough). This safety precaution will protect your equipment.
Thanks I will check with my Jeol Engineer about draining the air compressor, Currently,I am using JEOL JSM7100F FESEM.
@Ajay Rakkesh
Air compressor (if used) is not connected in any way to specimen chamber and has nothing to do with "collection" of impurities.
@Yashwanth Purushotham
There is no simple answer to your question. You'll always have outgassing when pumping out your microscope with specimen in; it is not dangerous in most cases. Avoid oily, greasy and partially polymerized things. And no kid's clay in your instrument.
As Domenico said, it could be beneficial to treat a questionable specimen with vacuum. If pumping time is longer than usual, you may have a problem.
Thank for the valuable guidelines please suggest me the best book to understand electron optics and electron microscopy.
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