20 January 2021 1 9K Report

Currently Iam working with Bacillus atrophaeus and Geobacillus steraothermophilus spore production. I need a suggesition regarding the media composition which could result in high yeild and under a shorter incubation period. Kindly let me know if any suggesions with respective reference articles on this regard.

More Yashwanth Goudu's questions See All
How to safeguard our FESEM or TEM instrument from unknown sample releasing impurity while analysis

In electronic microscope the we need high vacuum to attain high quality images. How to safeguard our FESEM or TEM instrument from unknown sample releasing impurity while analysis? Where as the...

18 July 2017 9,566 5 View

Is octane number of a compound related to isomerism, if yes how?

For example, n-butanol, and iso-butanol have different octane numbers. What is the influencing factor behind different ON, though both of them have the same chemical formula.

24 January 2016 2,896 2 View

Similar questions and discussions
Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View

Can someone help me get this article?

..?

15 June 2024 6,072 2 View

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method?

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method other than cryopreservation of spore for a short term...

10 June 2024 4,992 1 View

What is the correct way to measure the lengths of curved spores?

Measuring the lengths of curved spores sometimes makes confusions. Here in the attached image I have marked two paths (i, ii) the length can be measured. Hope an expert can suggest the correct way.

08 June 2024 1,109 5 View

How to start purification of enzyme from bacillus licheniformis as I am not getting clear supernatent after centrifugation it feels like jelly in it?

This jelly gets precipitated during ammonium sulfate precipitation

06 June 2024 5,631 4 View

Can anyone offer advice on how to show the inhibition zone of bacillus against s.aureus?

Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...

26 May 2024 2,151 6 View

Which long preservation method(s) can I use for Stenocarpella maydis also Diplodia maydis? So that I remains intact and does not loose traits?

I am Senior Research Technician and I have some isolates of Stenocarpella maydis also Diplodia maydis. I am currently try to develop SOP for long term preservation, so that it does not loose its...

06 May 2024 5,809 4 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close