How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View

How to calculate visual angle of a visual stimulus?

I am currently trying to figure out how to calculate the visual angle of some visual stimuli I used in an experiment. I think I have all of the information I need to calculate this. One is a...

15 February 2021 3,189 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Is a Strong correlation between the Moderator variable and the dependent variable an issue for moderation model (model 1) in Hayes PROCESS?

In a typical moderation model with one moderator, is it a problem if the moderator and the dependent variable show a strong correlation (say 0.85)? What if a strong correlation exists between the...

27 January 2021 4,937 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

Gear reduction for back-drivability of a motor?

Hi everyone, Does anyone know up to what reduction ratio of gear does a motor remain back-drivable? I want my robot to have safe interactions with the environment and other humans. Can you give a...

26 December 2020 7,142 3 View

How to export DigSilent Plots with high quality?

Hi all; I've started using DigSilent these days. However, I'm wondering how can I export the results with high quality the same as MATLAB plots as I currently do. Thanks

19 December 2020 2,666 2 View

How to assign initial stress to a coating in Abaqus?

Hello! I am trying to define/assign initial stress to a coating I modeled on a substrate. I defined the coating using "skin" function. I have tried "predefine filed" in load section. But it only...

10 December 2020 2,921 8 View

How to model a multilayer coating in ABAQUS?

Hello! I'm trying to model a multi-layer coating on a substrate in abaqus. The layers are homogenous and isotropic. My question is how should I approach the modeling? WHAT I'VE TRIED SO FAR: I...

09 December 2020 1,235 3 View

How can I calculate Nusselt number by using this equation (Nu = ht.gradTx * nx + ht.gradTy * ny + ht.gradTz * nz) step by step in COMSOL?

Calculate the Nusselt number using the COMSOL Program

09 December 2020 3,354 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

References for relative humidity created by salt solutions at different temperatures?

I understand that the ability of standard salt solutions commonly used to produce a certain relative humidity (RH) in a closed space changes somewhat with the temperature. Where do I find data...

04 February 2021 7,370 4 View

Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View

How to create a scaffold by using the particle leaching process?

In the leaching process,Why does NaCl settle despite sieving? How can we understand how much solvent the system needs? And, how can enhance the mechanical strength in this process and find the...

12 January 2021 5,679 2 View

How to get mesoporous cobalt ferrite thin film on Pt/Si substrate by electrodeposition method?

We want to deposit a thin film of mesoporous cobalt ferrite using a surfactant on Pt coated Si substrate by electrodeposition. What is the procedure for it? Does KLE polymer useful for this procedure?

10 January 2021 467 10 View

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture??

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture. I got poked after sprayed them with 70 % alcohol and dried them right away. Is...

07 January 2021 9,412 2 View