Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is iron oxide (rust) a problem in DNA extractions?

Hello, I just started extracting DNA from some spiders, using 5-mm ball bearings to grind the specimens in a tissue mill. I had ordered the ball bearings on Amazon, and thought it was a good idea...

21 July 2020 2,271 6 View

Comparing two variables to one variable- is this possible in SPSS?

In a quantitative study, three variables-- I am comparing two variables (self-concept & well being) with another variable (intent to leave profession). I am predicting to see a positive...

05 May 2020 6,362 3 View

Is there a human gut microbiome database that shows normal variability as well as pathological vs normal ranges for specific microbes?

When a diagnostic lab furnishes normal ranges for certain microbes, is there any place to compare their ranges to what scientists are discovering is normal variability or adaptations? Is there a...

29 December 2019 4,771 2 View

Is it imperative in psychology research to state the null hypothesis?

I have had feedback from a reviewer that I need to restate my hypotheses as null hypotheses. My supervisor disagrees with this suggestion. Having done a bit of digging, I found Szucs &...

12 December 2019 1,447 4 View

How to fit data with binomial distribution?

Hello researchers, Which program or software works best to fit data with binomial distribution? Many thanks, Neena

15 November 2019 6,609 3 View

Stereochemistry Check for Peptides?

Hello researchers, Is PROCHECK the correct tool to check the stereochemical quality of small peptides? My peptide of interest: polypeptide with 16 alanine residues. Many thanks, Neena

28 October 2019 4,653 2 View

I have a GST tagged fusion protein in soluble phase will it have folded?

Normally this protein EIF4E is expressed untagged and expresses in inclusion bodies that are refolded through dialysis, but its very long winded and likes to precipitate during dialysis resulting...

11 June 2019 622 3 View

I am in the process of recruiting participants for my PhD using IPA and am now re-thinking the data collection design. Can you give me any advice?

28 April 2019 8,737 4 View

Approx. how much IL10 do activated T cells secrete after 48 hours in culture?

My students are culturing mixed mouse splenocytes in culture for 48 hours with anti-CD3/28. We are then going to analyse for the presence of Tregs and measure IL10 secretion by ELISA. In order to...

20 March 2019 5,857 3 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View