Blood coagulation requires Ca2+, in blood bags citrate is present to sequester the calcium ions and thereby prevent coagulation ( http://ilovepathology.com/blood-bags-types-and-uses/ ). You do not need to remove the anticoagulant - just add calcium chloride solution to initiate clotting.
Dear Irza Mahmood . Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the resulting supernatant, designated serum, is carefully removed using a Pasteur pipette. See the link: https://www.thermofisher.com/in/en/home/references/protocols/cell-and-tissue-analysis/elisa-protocol/elisa-sample-preparation-protocols/plasma-and-serum-preparation.html
Acid citrate dextrose is the most commonly used anticoagulant to store blood in the blood banks as it prevents coagulation by inhibiting the action of the calcium ions. See the link: https://www.vedantu.com/question-answer/the-anticoagulant-most-commonly-used-to-store-class-9-biology-cbse-5fa21ed7e5a3730cf2caf767