How to release and study the activity of peptide encapsulated in chitosan nanoparticles?

06 June 2016 1 10K Report

The peptides are multi-functional and hydrophillic

To measure the release of peptide from nanoparticles, you can pellet the nanoparticles in a high-speed centrifuge and take the supernatant, which will contain any peptide that has been released.

Alternatively, you can use a centrifugal ultrafiltration device with a large molecular weight cutoff that will let the peptide through but hold up the nanoparticles.

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