What are the "units" in RNA seq data processed by RSEM?

Hello! I've been using gene expression data from the TCGA, where the RNAseq is processed using RSEM. I did a few graphs such as boxplots showing the expression of different genes in normal vs...

14 October 2020 2,721 2 View

Which is best way to purify my His-tagged protein after eluting from a Ni NTA column in denaturing conditions (urea 8M)?

I have cloned two versions of my protein of interest to produce antibodies in mice. It is not soluble in native conditions, so I am trying to purify it in denaturing conditions with urea 8M. Now,...

18 February 2020 7,305 3 View

Multi-layer wells "robbed" of pumping rates?

By the way, I send attached the workflow I've performed. It is strange that in the Link to parameters window, in the Topology processing, instead of "no topology processing", now what I get is "no...

17 February 2020 8,662 3 View

Is there an easy way to count the diatoms in a rock sample in a short amount of time?

Hello! I have a bit of a struggle with my diatomites samples. I have tried all the methods to break the diatoms from the rock but I got a negative results (they are highly silicified) and I want...

04 September 2018 6,353 3 View

Any recommended (free) software to rectify and post-process coastal oblique images?

We have a fixed time-lapse camera (RGB) located on a hill oriented towards a sandy coastal area. Images are taken every 30 min. By using a set of ground control points (GPS) we aim to rectify and...

24 October 2017 4,632 5 View

Does Pfu Taq polymerase generate 5'P end?

Does the PCR product amplified using pfu polymerase have 5' phosphate groups to use for blunt cloning?

22 June 2016 1,058 3 View

How can I break the exopolysaccharide capsule of the mucous colonies to extract the RNA and DNA?

Hello to everyone! I'm working with strains of Acinetobacter baumannii resuspended in NaCl 0.45% (8 McF in 2 mL). Colonies of Acinetobacter baumannii are mucous so that they can not be separated...

15 June 2016 6,500 1 View

Why do I have a smear in gel for my bacterial RNA sample?

I have a question.....i have made a agarose gel of bacterial RNA and i have obtained a smear....what's happened? the RNA is 588mer. Moreover, i have done a Real Time PCR of these RNA without...

25 October 2015 7,189 4 View

Large-scale reaction with TnT T7 quick coupled transcription translation system (Promega)?

I wonder if the TnT T7 quick coupled transcription translation system (Promega) works in a large-scale reaction. They suggest a final volume of 50 ul and I would need a final volume reaction of...

17 August 2015 8,270 1 View

What is the best protocol of histones isolation from bone marrow?

I am planning to analyze post-translational modifications, including acetylation and methylation of human histones, H3 and H4, via mass spectrometry (LC-ESI-Q-ToF). Can I ask for some advice and...

29 June 2014 8,633 2 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View