Whenever I attempt to transfect P. falciparum parasites I always get a time constant value of 6000 ms or higher, never in the range of 7-11 ms as mentioned in all the literature.
Apparatus - Gene Pulser II
Settings - 2 mm cuvette, 0.31 kV, 950 mF,
I have followed the published protocol to the letter and even have changed the water to deionised water (from invitrogen) for preparing the cytomix. However till date there is no result. How can this problem be solved?
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