I am running standard VFA samples, but the gap between each peak is wider. I changed the column with a new one and still have the same issue. The initial oven Temp is 110c, then the next 130c (ramp 1), then the maximum goes up to 200c (ramp 2). Total gas flow is set to 30ml/min.
I have used the same methods before and always got consistent results with less gap between the peaks.
Need to change the rate of temperature increase. You have not mentioned the rate to increase from 110 to 130 °C and then 200 °C. So it is difficult to tell what should be the rate? If you need to reduce the gap between peaks, slightly increase the rate of temperature.
Aditi Kundu thank you for your reply, for more clear understanding I am attaching a picture of the current oven setting along with the graph that I received from the recent run of standard samples of Volatile Fatty Acids. Peaks are in decreasing order. I will be more than happy to have your suggestion in this regard. Thank you.
The effectiveness of these strategies can vary depending on a patericular chromatographic conditions, the nature of the sample, and the equipment used. Therefore, it will require some experimentation and optimization to achieve the best results for your particular analysis.
In Gas Chromatography (GC), the gap referred to as the "valley" between peaks. Reducing the valley between peaks is important for obtaining better resolution and more accurate quantification of individual compounds. Here are some strategies to reduce the gap between two peaks in GC:
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