Hi researchers,
I am working on entomopathogenic fungi Cordyceps and Metarhizium. So i need to rear Setora nitens (Lepidotera: Limacodidae) for patogenity test on Lab. Currenly if i collect pupa from field, its can hatched to adult and then mating, produce many egg, but when its hatched to first instar larve its died in few days. And when i collect larvae from field its died on second day in Lab. If any one have experience working on it, please share method with me. Thanks
According to the diagnosis and symptomatology that you presented to us, most likely your Setora nitens caterpillars are dying by a baculovirus infection. To solve this problem you must collect tender leaves of oil palm free of viruses. Disinfect the leaves with a 10% solution of sodium hypochlorite, rinse and feed the larvae. Keep the larvae in clean ventilated cages with good space.
initially ascertain the cause of death of the larvae (lab reared and field collected) as the insects may be infected with micorbial such as bacteria, virus etc that could pass horizontally and vertically from generation to generation.
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