Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Are there any papers that deal with the effects of climate change on the pollination in Tropical orchids?

There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.

04 February 2021 1,818 4 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Forced Vibration Testing using FDD?

Hi, Can we use FDD algorithm for forced vibration response (sinusoidal response produced from eccentric mass vibrator)? Thanks

22 January 2021 5,851 2 View

How to modify micro-structure of Pure Copper which is available as a flat plate of 25 X 25 sq. cm?

Hi everyone, for a thesis project I have to modify the micro-structural properties of pure Cu flat plate of dimension 25 X 25 sq. cm . As I tried searching the available literature, I found a lot...

09 February 2021 5,090 3 View

What is the most efficient way to generate amorphous silica in LAMMPS?

I would like to generate multiple large samples of amorphous silica in LAMMPS. Currently I am generating it by beginning with a lattice of silicon and oxygen atoms and using a melting and...

28 January 2021 6,128 4 View

Is it possible to economically reuse the heat content of water vapour that is generated during quenching of continuous casting of steel?

A huge amount of water is evaporated to cool down and solidify the liquid metal strands coming out of bottom of CCM mould. The steam contains significant latent and sensible heat energy, besides...

26 January 2021 3,446 4 View

How to study pyridine anchoring mode on TiO2 by ATR?

I want to study the anchoring mode of a pyridine dye on TiO2 nanoparticles. I searched and a lot of papers study that by FTIR using by the attenuated total reflectance (ATR) method. I tried to...

12 January 2021 5,915 3 View

Does phenol red quench the light emitted by the scintillator in radio active assays?

I would really appreciate it if someone has experience with radioactive assays and could help me troubleshoot my experiment a bit. I could not find any literature evidence, but I am suspecting...

10 December 2020 4,810 3 View

Why I see no changes in hardness of AlCu alloy after 2 months natural aging?

08 December 2020 2,929 4 View

What is the effect of isothermal heat treatment on materials structure from room temperature?

Hi all I would like to study the microstructure evolution of semi-solid tin-lead alloy for an extruded sample during the isothermal heat-treatment process. Is there any difference if I heat up...

18 November 2020 9,087 7 View

Does Vectashield really quench Alexa Fluor 647 fluorescence?

If yes, when do you start to see the effect? Within hours of mounting or several days? Is it case specific? I have had nice stainings with several synaptic proteins/markers when using AF647 in...

13 September 2020 2,464 1 View

How can I properly quench POCl3?

Hey! I was wondering if there is a procedure that details how to properly quench a POCl3 solution. If you have any advice on how you efficiently conducted this process, please feel free to...

13 September 2020 3,605 4 View

What is the suitable method to find out the DC conductivity of glass and hence activation energy for the conduction, experimentally?

I am preparing oxide glasses (Silicon-phosphate) by melting and quenching method. I want to measure DC conductivity of the glass obtained after annealing. I am trying with Van der Pauw Four probe...

27 August 2020 922 3 View