Dear all,
I want to record some raman spectra of beer turbidity particles like polyphenols, proteins and polyphenol-protein conjugates with raman microspectroscopy at 535nm. Unfortunately I only get a strong fluorescence noise without any peaks. I tried to use AuNps fixed on a Indium-tinn-oxide coated microscope slice to quench the fluorescence but I only got the same bad results. Is it possible that the distance between the AuNPs and the analyte is too large for successfull fluorescence quenching? Would it be better to mix the AuNP dispersion with the analyte first an then let it dry on the microscope slice? Any other ideas?
Greetings
Joseph
Dear Joseph,
Can you measure the absorption and fluorescence spectra of your beer turbidity pariticles first? Then you know where the absorption and fluorescence peaks are. Therefore you can choose some filters to avoid the strong fluorescence by cutting off the photons absorbed in the sample. It is difficult to introduce a dopant to quench the fluorescence from your sample, which could lead to extra problem from dopants.
The background in Raman can also come from scattering, of the source laser and the Raman signal itself. For strongly scattering, inhomogeneous samples, you should ideally work in strict confocal conditions. Sonicating may help.
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