I am doing staining for additional markers on sections from mouse expressing the mTmE reporter. The endogenous tomato red signal is unnecessary in this current experiment and in fact, complicating my staining (because I also have to stain GFP, thus the additional marker was label with far-red second antibody--which turned out too weak to be inconveniencing).
Dont know the answer to this problem. Mike Mancini might know. Sorry I cant be of help.
Austin
Hello Yi, I am new to this forum. Were you able to find a solution to quench the tomato fluorescence and still achieve good staining with other antibodies?
Check this paper from Lin et al. on muliplex immunohistochemistry
http://www.nature.com/articles/ncomms9390
You can find many other methods on this line.
I didn't tryed none of them yet but this one looks simple, convincing and promising.
I'll let you know!
Federico
http://www.nature.com/articles/ncomms9390
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