Dear Sankha Karmakar,

Colorimetric determination of pectin substances can be carried out using the procedure depicted in the following article:

E. A. McComb, R. M. McCready

Anal. Chem., 1952, 24 (10), pp 1630–1632

DOI: 10.1021/ac60070a036

Publication Date: October 1952

Hoping this will be helpful,

Rafik

To quantify pectin from natural sources you can use m-hydroxyphenyl colorimetric method. It gives the uronic acid concentration, than you need to extrapolate to pectin content. fillsetti-cozzi & carpita 1991. Analytical biochemistry 197;157-162.

Maybe https://www.researchgate.net/profile/Adamara_Nascimento or co-workers can suggest a more updated method.

m-hydroxydiphenyl