Why does the band sizes on Agarose gel electrophoresis differ from the peaks at capillary electrophoresis?

14 February 2021 4,773 4 View

Can we find receiver sensitivity using MATLAB simulation?

I have a MATLAB code for point to point based new LPWAN system and evaluated its BER performance. Can I use the same code with some modifications to find its sensitivity?

11 January 2021 1,374 3 View

What was your best and worst experience of working on a research paper during LOCKDOWN/COVID19?

Working during pandemic was the most challenging task I have ever seen. I will be sharing my experience and research output during lockdown in Indian scenario, do share your experience and...

25 December 2020 3,072 7 View

Calculation of prepare 1:1 CuFe2O4/Ppyrrole nanocomposite?

Recently., we make the supercapacitor cell from nanocomposite Can any one tell me the volume of pyrrole to make 1:1 CuFe2O4/Ppyrrole nanocomposite thanks in deed

19 November 2020 9,572 7 View

How to calculate the magnetic nanoparticle size from magnetization data which can be carried out by VSM?

How to calculate the magnetic nanoparticle size from magnetization data which can be carried out by VSM?

05 October 2020 8,547 4 View

What is the best binary metals oxide nanoparticles for to generate Solar cell? and the best value of energy gap ?

what are the best binary metals oxide for to generate Solar cell? and the best value of energy gap ?

24 August 2020 8,853 7 View

How to define a direction to the topology optimization in Comsol?

I am doing a topology optimization of heat transfer problem. The results give a good correlation to the optimization goal. Yet, if we take a 2D-axisymetrric geomerty (attached). The result of...

26 July 2020 2,690 5 View

My melanoma cell line doesn't grow well suddenly, May i know the possible reasons for that?

I have 92-1 of melanoma cell line , when i thawed them they were totally normal , but after first passage , the cells begun not growing well and not totally attaching on the bottom of the flask...

30 June 2020 3,622 4 View

How to model water in Abaqus?

Hi everyone, I want to model water on the upstream of the dam. Is it possible in Abaqus or any other software?

15 May 2020 8,954 13 View

How can i model spherical gold nanoparticle in scaps-1d?

I am studying and doing research on the solar cell with different nanoparticles using SCAPS-1D for simulation. Can I model spherical, conical and cylindrical shape nanoparticles in SCAPS-1D?

27 April 2020 7,298 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View