Hey everyone,

I am stumped on this and hope you guys can help!

I am trying to quantify some data I have with actin. I have treated neurons with a drug that drastically changed the actin cytoskeleton to lose projects and almost round around the nucleus rather than the straight filamentous structure we observe. I am having a difficult time quanifying this, because I do not observe any changes really to the overall levels. I wanted to quantify "roundness" but this has become a challenge because neurons love to be very close to one another and a lot of the ImageJ plugins really need single cells, but if I plated the neurons so sparse they would just die. Does anyone have any suggestions?

Thank you!