I try to concentrate and purify an antigen from cell culture medium, which is a cleaved product from a transmembrane protein. My plan is to use an antibody to pull down the antigen through IP and elute the antigen only. As I don't want to elute the antibody as well, so probably I need to cross link the antibody to A/G agarose beads.
1) Could you get me any suggestion about the cross-link method? Or any other ways to prevent antibodies from being eluted?
2) What kinds of buffer will be suitable for eluting my antigen from the complex without denaturing it?
Thanks a lot!
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