Regarding Topology and coordinates of ligand molecule?

Although, I m getting results with Autodock 4.2, but further, to strengthen my results I am trying to use Swissdock, but every time the website reported the topology of ligand is not correct. Till...

26 December 2019 9,174 3 View

Multiple Regression with many independent categorical variables

Can many independent categorical variables be included in regression at once to predict the dependent variable. For eg: Dependent Variable = Income Independent Variable = Age (4 categories),...

30 June 2017 1,518 8 View

Hello friends, which is the best method to determine water content in the gummies???? how to prepare gummy sample for water determination?

07 March 2017 1,758 3 View

Do human fibroblasts get transformed on S.C injection into mice?

I have injected human fibroblasts into mice. On harvesting the tumors, will I have a combination of mouse and human fibroblasts or human fibroblasts transformed into mouse like. How can they be...

23 November 2014 1,368 5 View

What is a good method for quantification of cell invasion in Organotypic cultures?

see above

20 February 2014 8,792 1 View

How can we identify the Martensitic and Austinitic phase in Magnetic Alloys?

In Heusler alloys

12 April 2012 334 11 View

Hello everyone i am using acid hydrolysis n DNSA (Miller 1959) for calculating fruit sugar is this valid n acceptable?

01 January 1970 8,128 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Shortcomings or consequences of biological synthesis of silver nanoparticles?

Here I want to ask about the shortcomings and consequences of the biological synthesis of nanomaterials. Especially plant and microbial synthesis. Suggest me some reference paper. Thank you

20 December 2020 8,987 3 View

How much the microbial exploration in oil and gas industry is applicable?

Microbial oil and gas prospecting (MPOG) I would grateful it if you could provide me with your invaluable comments and ideas on the following questions: 1- Do you consider MPOG as a promising...

15 December 2020 9,895 2 View