Dear colleague Manoj

In the book: In vitro culture of Mycorrhizas (S. Declerck, D.G. Strullu, J.A. Fortin, eds.) published in 2005, Part VII Methodology. Section 6 AMFungal Propagules: Selection and Disinfection, 6.2 Disinfection Process, page 357. ISSN 1613-3382. ISBN-10 3-540-24027-6 Springer Berlin Heidelberg New York.

According to these authors, the disinfection occurs in two main steps: a chloramine T treatment (step 1) and an antibiotic treatment (step 2). Prior to the disinfection process and depending on the propagule, i.e. spore or root fragment, and on the complexity of its structures, e.g. ornamented spores which influence

the level of contamination, it is necessary to resort to pre-treatment steps

or to adapt the duration of contact with each solution.

Drying under open sunlight , at least for 7 days, is also the best way. I have practised this method in my experiment. Before drying, soil should be made fine breaking the lumps of soil and sorting any kind of roots or others plant parts with a seive. Special care should be taken not to contaminate anyway from airflow or any otherway. A polythene sheet is better for spreading the soil and again to cover the soil.

Dear Friends,

First treated the isolated spore with chloramine T followed by an Antibiotic solution and then washed them with autoclaved water. The results were satisfactory. Thank you all for your positive and informative comments.