When we are bio-synthesizing the nano-nutrients, it is naturally encapsulated by mother protein. To our experience we find that it is relatively more stable when we keep it in the refrigerator at 4 degree centigrade. The problem is that we are now in a position to commercialize the nano-nutrients but Indian farmers mostly dont have the facilities for refrigerator so they are mostly keeping outside for a longer time at 25-35 degree centigrade and that way with time protein layer brakes and particles were agglomerated and becomes less effective. If anybody suggest some way to protect the protein layer of bio-synthesized nano-nutrients so that farmers can adopt it.
It is a delicate material. It is not my area of specialization and not aware about sensitivity. Can not we keep them in icebox for storage at farmers level.
proteins are best stored at ≤ 4°C in clean, autoclaved glassware or polypropylene tubes. Storage at room
temperature often leads to protein degradation and/or inactivity, commonly as a result of microbial growth. For short term
storage (1 day to a few weeks), many proteins may be stored in simple buffers at 4°C. Protein Stabilizing Cocktail (Product
No. 89806) is a 4X solution that helps to extend the shelf-life of most proteins for storage at 4° C or -20°C compared to
storage in simple phosphate or Tris buffers.
For long term storage for 1 month to 1 year, some researchers choose to bead single-use aliquots of the protein in liquid
nitrogen for storage in clean plastic containers under liquid nitrogen. This method involves adding the protein solution drop-
wise (about 100 µl each) into a pool of liquid nitrogen, then collecting the drop-sized frozen beads and storing them in
cryovials under liquid nitrogen.
Frozen at -20°C or -80°C is the more common form of cold protein storage. Because freeze-thaw cycles decrease protein
stability, samples for frozen storage are best dispensed and prepared in single-use aliquots so that, once thawed, the protein
solution will not have to be refrozen. Alternatively, addition of 50% glycerol or ethylene glycol (see Additives section below)
will prevent solutions from freezing at -20°C, enabling repeated use from a single stock without warming
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