Hi,
I am conducting a experiment where I am attaching fibroblast cells to a PEDOT:PSS film surface. Unfortunately the cells are more attracted to the surface of the 24 well plate in which the PEDOT film is located.
Is there a simple coating I can apply to the PEDOT film to increase cell adhesion or is there a coating I can apply to the 24 well plate to inhibit cell adhesion.
I have looked at using bovine serum albumin or Angiogenin, but using proteins are very expensive to coat surfaces in. So far poly-l-lysine seems like the front runner for increasing cell adhesion to the PEDOT film.
I would appreciate any other suggestions.
Good day, Mohammed!
Probably, the simple thing is to cover the film with collagen, fibroblasts adhere very well and grow on collagen.
Hi Mohammed,
As Alexander above, and you previously, suggested pll and collagen will very likely improve the adhesion of fibroblast. However, may also try seeding the fibroblast on the film as a "drop culture" and just add more media to the well afte cells have become attached to it?
Cheers,
Mohammad,
The answer is very simple: serum, the same stuff you use to propagate your cells.
How to stop them attaching to the culture dish?
1. don't use TC plastic! Use raw polystyrene (microbiological plastic)
2. coat your TC plastic with 1% BSA (or non-fat milk)
3. lay your PEDOT:PSS onto a sheet of Parafilm (sterilise it with 70% alcohol)
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