22 February 2020 6 4K Report

protochol of produce vitamine B from bacteria by properties probiotics

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Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Drawing fullerene more than 60 carbon?

I have ChemDraw 22.2.0 and I want to draw PC70BM but I just found C60 and less in the templates so, any one can help me ?

18 June 2024 3,098 3 View

Why do I get no melt curve peak and no Ct for some of my patients but not others, in a SYBR green qPCR?

I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...

31 May 2024 1,088 0 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Anyone with phosphate flotation experience?

I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...

14 May 2024 4,505 4 View

Why my amino acids do not contribute in amidation reaction?

Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...

15 April 2024 6,778 3 View

How to increase concentration and quality of RNA after extraction?

Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...

11 March 2024 9,171 8 View

How to interpret my samples' results from D1000 ScreenTape Assay?

Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...

10 March 2024 8,764 0 View

Can anyone help with SHSY5Y cells differntiation?

Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...

19 February 2024 1,672 5 View

Why Cryo cells (freezed cells) do not attached to the flask?

Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...

19 February 2024 9,305 2 View

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through genetic modification or metabolic engineering? Is it helpful, good for health ?

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How to ask to correct the Incorrect Claim regarding the writer of Article that I was involved in?

Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...

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What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

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Can all postbiotics survive the gastric journey or is it again strain-specific or will depend the type of postbiotic?

General understanding is that postbiotic particularly those that are heat-treated are stable to heat and their activity remains stable during gastric journey. Wondering if this is true for all...

25 June 2024 3,133 3 View

Did you ASK AI (https://talkai.info/tr/chat/) the same question?

What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...

22 June 2024 4,567 0 View

Can increasing bacterial concentration in a formulation compensate for inhibition by lactobacillus strain,and if so ,is it right to do so?

I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...

01 June 2024 338 2 View

What is the function of CaCl2 in the lipolytic assay? what percentage concentration of CaCl2 should be added in the lipolytic assay?

Hello everyone. I need some information about the use of CaCl2 in lipolytic assays. I often see the addition of CaCl2 in lipolytic activity assays, especially in probiotic screening, but there is...

08 May 2024 7,480 2 View

Is it good to have antibiotic resistance property for probiotic?

To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...

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