How to produce transmissible gastroenteritis coronavirus ?

More Joanna Zmurko's questions See All

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

Volcanic Ash in modern concrete and cementitious materials?

I am writing a paper summarizing my research results on the possibility of usage of volcanic ash in modern cementites materials on the example of the tests of composits with particular (Japanese)...

21 February 2024 5,797 0 View

How to calculate DBH for trees with multiple dead and live stems in the same tree?

I am trying to calculate DBH (diameter at breast height) in order to determine the demography and basal area of trees. However, some of the trees have multiple stems which makes it difficult to...

23 January 2024 845 0 View

ReserchGate misatributes some citations to me. What should I do?

The site shows that my works are cited in some papers, however when I checked those papers, I found no sign of being referenced (on the other hand my work is referenced in completely different...

22 January 2024 3,766 2 View

Are you interested to collaborate within the new research project "Education in the AI era"?

I'm looking for hard-working researchers for the new research project "EDUCATION IN THE AI ERA." I would like to create the survey on Google and do international research. If you are interested in...

06 January 2024 5,488 21 View

Why I'm losing plasmid after digest with restriction enzymes?

Hi everyone, I'm currently working with a plasmid called pBI101, I isolated this plasmid from a plate then I did the extraction with mini prep protocol and cut it with SmaI at 25°C incubation...

17 November 2023 6,980 3 View

How to desing “splinkerette” adapters for TraDIS (transposone) libraries?

I'm trying to optimize TraDIS for our model bacterium and I am having some technical problems with the DNA preparation for the sequencing step. I'm struggling with efficient PCR enrichment of...

12 September 2023 5,836 0 View

Transfection of adherent primary cells using Neon transfection system - the best electroporation settings to start with?

Hello everyone, I am trying to make a CRISPR-Cas9 knockout on lung cancer primary cells using the RNP complex. I am using the Neon Transfection System but so far it failed - all the cells that...

30 August 2023 8,660 2 View

BDNF unit conversion - does anyone know the formula?

Hi, does anyone know the formula for converting the BDNF value in biological fluids (pg/ml to ng/ml and vice versa)?

24 July 2023 1,280 1 View

How do I get factor loadings for single indicator latent variable in SEM?

Hi, I'm doing CFA using SPSS Amos and I have two variables with single items each. After reviewing previous discussions, I've fix the loadings and error variance to 1,1. If I put error variance to...

24 June 2023 3,631 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?

27 July 2024 5,916 1 View

Is a reliability test necessary in my survey on translations?

Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...

24 July 2024 8,245 5 View

Can you suggest reliable procedures to get displacements from accelerations in frequency domain ?

I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.

21 July 2024 3,465 5 View

What is the Scopus and Beall's dilemma?

I've found that some journals are both Scopus-indexed and listed on Beall's list as predatory or potentially predatory. Why does this discrepancy occur? Are there any more reliable platforms than...

12 July 2024 5,158 1 View

Are open access journals reliable and difference between an open-access journal and a paywall journal?

12 July 2024 8,971 2 View

Yin Zhe

Unrelated to the virus, the key is infectivity. When self-healing environmental DNA, mistakenly think that the infection process is the self-healing behavior of the adaptive environment. Any infection is the natural law of biology. and the survival process.

David Escors

Hi, use swine testicle cells (ST) until they reach confluence, and then let the cultures grow old for a few days, until the medium is very acidic. This by some reason helps the TGEV to reach high titers. Then, infect the cultures with an moi of 0.5 and let the infection proceeds until a 100% cytopathic effect.