How to produce bioethanol from brown seaweeds using bacteria or yeasts?

18 October 2016 1 8K Report

lab scale procedure is needed.

Bioethanol production from brown seaweed, Undaria pinnatifida, using NaCl acclimated yeast

https://www.researchgate.net/publication/235382529_Bioethanol_production_from_brown_seaweed_Undaria_pinnatifida_using_NaCl_acclimated_yeast

Article Bioethanol production from brown seaweed, Undaria pinnatifid...

Badges
Science topic

More Shruti Shete's questions See All

Doping of metal on noble metal nanoparticles?

can we dope any metal (Cu, Zn, Mg) on silver or gold nanoparticles and how can it be done ?

06 May 2024 5,953 3 View

How do i calculate sample size for a survey questionnaire of my research?

I am working on tobacco intervention and developing communication strategy, for that a questionnaire was developed, population is Indian tobacco users.

13 April 2024 9,324 2 View

What is accurate methode to set up two electrode device in supercapcitor device?

I made two electrode devices for the supercapacitor. I combined activated carbon and PVDF for negative electrodes in an 80:10:10 ratio. I combined active working material, PVDF, and activated...

12 February 2024 2,964 3 View

How to import file on xpspeak41?

I want to analyse XPS spectra from xpspeak41. I try every file format such as ASC, .dat but it always shows an error reading file. what should I do? please see the images I attached below

11 February 2024 6,860 3 View

How can I make different concentrations of antigen solution from a stock?

I have 50 ug of Human Her2 / ErbB2 Protein, Tag Free antigen in white powder form and need to make various concentrations of that antigen, such as 50 unit/ml, 10 unit/ml, 0.01 unit/ml, and 0.1...

05 February 2024 9,048 6 View

Can someone please help me with optimized protocol for intracellular melatonin detection using fluorescence spectroscopy ?

My work involves the production of melatonin using saccharomyces cerevisiae and its quantification. I have tried various fluorescence spectroscopic methods but couldn't find an optimized protocol...

15 January 2024 9,410 3 View

I want to design primers for RT-PCR, how many primers can be multiplexed in one go?

For validation studies, I want to use RT-PCR for genes derived from sequencing data. For that I have to multiplex the reaction to see the expression for more than one gene. How many primers can be...

18 December 2023 8,387 1 View

How to quantify intracellular melatonin without using HPLC or Mass Spectroscopy ?

I am currently working on yeast cells for melatonin production which requires me to quantify melatonin easily and economically. I was hoping that anyone could provide me with an optimized protocol.

09 December 2023 7,037 1 View

How can I speed up the growth of single MCF-7 clones after cell sorting?

I recently did a single cell sort with MCF-7 cells to create my CRISPR cell line. I sorted single cells in a 96-well plate in MEM media with 10% FBS, 10ug/mL insulin, and 1mM sodium pyruvate. It...

28 October 2023 6,320 1 View

Why is there often a difference in the values of specific capacitance measured using Cyclic voltammetry and GCD? Which value should I use?

Which value is more accurate and why?

17 October 2023 1,418 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View