I the past we worked with dual media. e,g. by growing the fungus on rich medium and allowing it to cross a barrier with low nutritional value. Poor plate with one medium, let slodify cut out half and poor the other medium in the occuring empty halve.

Thank you. Interesting suggestion Cees and I will try it. Which media did you use (rich and poor)?. I guess once you have the dual medium solidified you inoculated fungus on far end of the rich medium and let it grow towards the poor medium. Are there any specifics re incubation conditions you used that supports the fruit body production?

When it comes to polypores, the best Method is to give cultures daylight and oxygen, not worrying for contamination. The fungal "immune system" takes care of it.

We have used a modified medium (nutrient rich) that is allowed to solidify in the middle of a petri dish, and the other half filled with water agar 1.5%, the initial inoculum goes on enriched media, and then fungus over the water agar is allowed.

Thanks Beatriz Elena. That seem to be in accordance with what Cees Waalwijk is saying although he has two different media in two halves of a plate. Have you, in addition, played with using black and grow light and any other shocks (temperature differential, flush with water, humidity changes...)?

Please refer to the method we used which was published in Journal of Tropical Forest Science 11(1): 240-254 (1999).

We transfered small discs of the fungus into debarked sterile wood blocks in autoclavable plastic bags supplemented with 2% malt agar.The stoppered bags were incubated at room temperature in the dark to allow good colonization of the wood blocks by the mycelium - this could take up to about 2 months. The well colonised blocks were then removed from the plastic bags and "planted" to one third of their depth into unsterilsed garden soil, one block per bag/pot. The bags were kept in the shade house and lightly sprayed with water daily to keep the soil and the wood blocks moist. Sporocarps were produced between 2-3 weeks after 'planting'.

Why don't you separate the process? basidiospore formation may be achieved with your method but mycelium or spore is enough to grow a fruit body (I assume you meant sporocarp). You may experiment with alternate light and dark, persistent dark and persistent light. The biological potential of the inoculum is barely enough to support fruit body production which can be achieved with a body of suitable substrate determined by whether the basidiomycetes is lignoclous or cellulosic. Serach literature for more infor.

Thank you Omorefosa

Yes this will further help in adjusting existing protocols. Many thanks Nyoman. Adnan

Since spore or basidiospore is usually formed and obtained from the fruiting body (pileus) of a white-rot fungi, would it still be illogical to obtain same spores from a mycelial culture of it in the laboratory (grown on solid agar, as is found in some literature? Plate culture are often said to sporulating, is this still applicable to mushroom or other basidiomycete? Thank you. 

Thanks for your answer and coments! I'll try it.