Sometimes during whole rat liver decellularization, we encounter patches of grossly incomplete decellularization, so we discard these samples. I assume that this is due to variation in resistance and therefore uneven flow of detergents (also kinking and liver position). We perform heparinizaiton before starting and we use low conc SDS as the main detergent. We also use a fixed flow-rate rather than fixed pressure perfusion.

I would be grateful if you can recommend a technical tip that can prevent this from happening.

Thank you,