in order to conjugate an antibody to a cytotoxic drug, I wan to use SMCC linker. I have a two step reaction. in the first step i want to conjugate SMCC linker to the antibody and in the second step conjugate drug to antibody-linker. SMCC is dissolved in DMSO. when I add linker solution to antibody solution, antibodies aggregate. DMSO is 5% v/v. I added the DMSO without linker and aggregation was also occurred but the aggregates were smaller. how can I prevent antibody aggregation during reaction?
Two approaches might be helpful (i) reducing the DMSO concentration; and (ii) diluting the antibody concentration. You may need some optimization of your first step.
Would you be willing to consider changing your crosslinker to sulfo-SMCC? it is water soluble, avoiding the need for DMSO.
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