09 September 2013 1 1K Report

I would like to do MALDI-TOF analysis of a few peptide samples. I have done tricine SDS-PAGE of the samples and would like to preserve them for MALDI-TOF analysis. How can I preserve the gel without damaging the peptides?

More Naveen Sathyan's questions See All
Has ResearchGate helped you tackle obstacles in your research?

RG provides you with a platform to discuss the problems you face in you research work with experts around the globe. It surely has helped me tackle many obstacles related to gene cloning. Has it...

02 March 2014 6,994 10 View

Which step do you find the toughest while attempting to write a scientific paper?

While attempting to write a paper everyone of us has a rough ride at one step or the other. Which one do you find the toughest....Deciding on the title, Introduction, analyzing the results,...

01 February 2014 6,528 10 View

Shouldn't journals abstain from publishing experiments on rare and/or endangered species?

Performing experiments on species with low population sizes raises serious concern as many experiments or studies require sacrificing the organism. Scope of publication in scientific journals...

01 February 2014 554 6 View

Can anyone identify this organism?

These creatures were found attached to leaves of mango tree. This is the first time I am seeing these. Can anyone identify them?

01 February 2014 2,622 20 View

What are the possible consequences of intense competitiveness in science?

In modern era, competition is becoming more and more intense. Does it encourage a scientist to put in greater effort toward solving problems or does it have a negative impact? In your opinion what...

31 December 2013 293 15 View

Shouldn't there be a platform to share negative results?

Generally 90% of a researcher's efforts end in negative results. The remaining 10% of his/her results are positive and it is shared with the world in the form of publications or patents. Negative...

31 December 2013 4,961 46 View

Is modern research becoming more and more “publication oriented”?

The main aim of research should be either to gain knowledge or to solve a problem and to work for healthy development of mankind and nature. Nowadays researches are planning their work, so as to...

11 December 2013 470 97 View

Why is it that bacteria feel "more at home" at reduced gravity?

Why is it that many bacterial strains become more virulent, have reduced lag phase and increased exponential growth rate when exposed to reduced gravity as in space stations? Does it have an...

10 November 2013 6,724 1 View

Can a phage make a bacteria more virulent?

Can a phage transform a non virulent bacteria into a virulent form. Are there any examples?

10 November 2013 5,893 8 View

Problems with liquid growth inhibition assay - can anyone help?

I am using 10 microlitre of test sample, 10 microlitre of microbial suspension (in hepes, adjusted to 1000 bacterial cells), and 80 microlitre of MH broth in a 96 well microtitre plate. Reading of...

10 November 2013 5,430 4 View

Similar questions and discussions
What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close