How to preserve Tricine SDS-PAGE for future analysis?

09 September 2013 1 1K Report

I would like to do MALDI-TOF analysis of a few peptide samples. I have done tricine SDS-PAGE of the samples and would like to preserve them for MALDI-TOF analysis. How can I preserve the gel without damaging the peptides?

Abhaypratap Vishwakarma

Pick the desired spot from your gel and transfer to fresh eppendorf along with Milli Q water. Store at 4 deg centigrade.

Badges
Science topic

More Naveen Sathyan's questions See All

Has ResearchGate helped you tackle obstacles in your research?

RG provides you with a platform to discuss the problems you face in you research work with experts around the globe. It surely has helped me tackle many obstacles related to gene cloning. Has it...

02 March 2014 6,872 10 View

Which step do you find the toughest while attempting to write a scientific paper?

While attempting to write a paper everyone of us has a rough ride at one step or the other. Which one do you find the toughest....Deciding on the title, Introduction, analyzing the results,...

01 February 2014 6,388 10 View

Shouldn't journals abstain from publishing experiments on rare and/or endangered species?

Performing experiments on species with low population sizes raises serious concern as many experiments or studies require sacrificing the organism. Scope of publication in scientific journals...

01 February 2014 425 6 View

Can anyone identify this organism?

These creatures were found attached to leaves of mango tree. This is the first time I am seeing these. Can anyone identify them?

01 February 2014 2,494 20 View

What are the possible consequences of intense competitiveness in science?

In modern era, competition is becoming more and more intense. Does it encourage a scientist to put in greater effort toward solving problems or does it have a negative impact? In your opinion what...

31 December 2013 181 15 View

Shouldn't there be a platform to share negative results?

Generally 90% of a researcher's efforts end in negative results. The remaining 10% of his/her results are positive and it is shared with the world in the form of publications or patents. Negative...

31 December 2013 4,838 46 View

No title

The main aim of research should be either to gain knowledge or to solve a problem and to work for healthy development of mankind and nature. Nowadays researches are planning their work, so as to...

11 December 2013 230 97 View

Why is it that bacteria feel "more at home" at reduced gravity?

Why is it that many bacterial strains become more virulent, have reduced lag phase and increased exponential growth rate when exposed to reduced gravity as in space stations? Does it have an...

10 November 2013 6,598 1 View

Can a phage make a bacteria more virulent?

Can a phage transform a non virulent bacteria into a virulent form. Are there any examples?

10 November 2013 5,745 8 View

Problems with liquid growth inhibition assay - can anyone help?

I am using 10 microlitre of test sample, 10 microlitre of microbial suspension (in hepes, adjusted to 1000 bacterial cells), and 80 microlitre of MH broth in a 96 well microtitre plate. Reading of...

10 November 2013 5,289 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View