How to prepare the stocks of 4-Nitrophenyl palmitate and 4-Nitrophenyl laurate to determine the Lipase activity of proteins?

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Re-solubalization of protein pellet after MeOH-CHCl3 precipitation?

I am doing the click reaction on the HEK293T cell lysate (200ug proteins) for 1h at R.T. After the click I perform the MeOH-CHCl3 precipitation, then I try to re-solubalize the pellet in 4% SDS...

01 August 2023 3,388 4 View

How are Cas13b gRNAs cloned into plasmid backbones?

When cloning gRNAs for any Cas protein, two oligos are required- the target sequence present in the actual mRNA/DNA and its reverse complement (the target sequence is then transcribed to produce...

02 July 2023 9,764 0 View

Why do we always consider linear range in electrochemical sensors?

While generating calibration plots for electrochemical sensors, we always take the linear range. Why cant we consider non-linear regimes?

25 September 2022 3,420 4 View

Quantity of curcumin dosage to impact on metabolism effect?

As I learned from various material, it seems regular consumption of turmeric approx 3 to 5 grams create an good impact to human bodies, I would like to know the amount of daily consumption of 100...

26 February 2020 1,161 4 View

Where can I find the recent population of Indian cities (2020s) ?

Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!

10 August 2024 857 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Challenges Faced by Freshers in IT Sector Recruitment?

i need to know what the key factors that the freshers are facing to get into the recruitment process, explain me clearly

31 July 2024 6,872 2 View

I am looking for the permission to use the OLBI survey. Does anyone know where I can obtain that information?

I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...

29 July 2024 3,684 0 View

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

What are the most effective strategies for integrating sustainability in your expertise?

What challenges do you think businesses or entities face in implementing these strategies? Thanks

28 July 2024 2,125 14 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View