When cloning gRNAs for any Cas protein, two oligos are required- the target sequence present in the actual mRNA/DNA and its reverse complement (the target sequence is then transcribed to produce the gRNA spacer sequence). These oligos need to have overhangs that correspond to restriction sites in the plasmid for cloning them. I have found online that when cloning gRNAs for Cas9, the target sequence is always cloned into the top strand and its reverse complement is cloned into the bottom strand, i.e., if a U6 promoter is in the 5'-3' direction, the target sequence is cloned into the 5'-3' strand downstream of the promoter. My question is- is this the same for Cas13b as well?