Dear Muhammad Abid Hasan Chowdhury, samples are prepared under reduced température, this is cryo-microtoming. For the tests look for those with informative information. My Regards

Hi dear Mohammad, I think you can use the conventional powder preparation method as follows:

First, the whole powder is stirred by a Spatula.

Then a small amount of the sample is added to a solution such as ethanol, isopropanol or acetone so that only the solution becomes becomes opaque.

The container containing opaque solution is placed in ultrasonic for at least 10 minutes.

After ultrasonic finishes, one or two drops of the sample are drip by dropper or pipette on a slide 1cm × 1cm.

After the sample is completely dried, the sample slide is placed on sample holder .

Hi Muhammad Abid Hasan Chowdhury !

I think FTIR helps you to identifiy functional groups of biomolecules capping your metallic nanoparticles

Thanks to all.