Nilofar Nilofar

4 Questions 11 Answers 0 Followers

Questions related from Nilofar Nilofar

How cells are counted in 96 wells plate by trepan blue assay?

usually, Trypan blue stain is applied before seeding cells in 96 wells plate and cells are count by hemocytometer. I am confused about how cells will be counted in 96 wells plate by trypan blue...

12 December 2019 2,597 4 View

How to calculate % yield of total polyphenol content?

I use 3 g dry sample for extraction and get 83 mg dry extract. from 83 mg I used 60 mg for folio-ciocalteu assay and get 60 mg/g TPC. I want to find the % yield of the TPC. anyone please help me...

08 August 2019 9,058 6 View

What is best value for Y in folin ciocalteu asaay?

For my 2 samples i am finding Polyphenol concentratiOn. For sample one: O.D of different concentration (high to low) are 1.777, 1.209, 0.915, 0.761. I select 0.761 for Y value and find 0.26 g/L...

07 July 2019 4,431 7 View

Can I simply filter and then dry the extract after microwave assisted extraction to find anti tumor activity of leaves extract?

Hello, I want to know after microwave assisted extraction, centrifugation is must? Or I directly filter and then dry the extract by rotary vacuum evaporator? I want to check the anticancer...

06 June 2019 9,506 3 View