How to interpret multiple bell shaped curves in Kratky plot?

Hi, I'm trying to analyze a Kratky plot generated from my SAXS data. I am seeing multiple bell-shaped curves in my Kratky plot and I am not able to find any source to get relevant info on this...

20 January 2021 2,474 1 View

Lift off problem with Indium/Gold deposition?

Hi, I'm doing 5/40nm In/Au e-beam evaporation at rates 0.2A/s(for In),1A/s(Au) on lithographically(doubly coated 495k,950k PMMA e-beam resist) done Si substrate at base pressure 2e-6torr. I'm...

18 January 2021 7,550 4 View

How to Import .inp file from abaqus CAE to Ansys perfectly?

I am trying to import an Abaqus .inp file to Ansys but it does not import the whole assembly but only imports a single part. How to solve this.

17 January 2021 5,450 3 View

Can I know the reason for these non specific bands?

a PCR and gel electrophoresis was carried out to obtain the results. they are negative jak2 v617f samples (ref genome - NG_009904 )

13 January 2021 7,658 4 View

Which third party CFD Codes can be used for FSI Co-Simulation in Abaqus ?

Since Abaqus CFD is no longer available in newer versions what are other available codes for Fluid-Structure Interaction Simulation

11 January 2021 2,012 3 View

How to determine Viscoelastic and Hyper Elastic Material Properties for Abaqus by mechanical testing ?

I am studying about Impact load resistance property of Polyurea (Elastomer). With respect to this, I need to conduct tests on this material to evaluate its material model behavior as a...

29 December 2020 2,969 1 View

How to remove rotation along z-axis and shift the origin to body's center?

I have attached the files with this post to explain me how to remove rotation and center the origin at the body's local coordinate. This is just one sample and I have 1000s of files like this. I...

18 December 2020 3,838 3 View

Reason for growth of fungus/mould in hydrothermal liquifaction filtered product of cellulose-based materials?

Performed hydrothermal liquefaction of cellulose powder, at about 200 degrees for 10 16 hours. After simple filtration, I stored filtered product at room temperature for 4-6 days, there is the...

03 December 2020 7,327 3 View

Is Coefficient of Variability (CV) positively correlated with root mean squared error (RMSE) in every case??

My data for fungicide treated replication shows higher RMSE and R-square but less CV compared to the untreated replication for a regression between vegetation indices and yield. How correct is it?...

23 November 2020 2,079 6 View

Fuel Cell not working after charging?

I am making an electric fuel-cell car using platinum as an electrode and sodium hydroxide as an electrolyte. I tried both series and parallel connection to charging it, Even after charging 2...

17 November 2020 2,860 9 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View