Hello Afaf Hadjer Kherief

You need to provide more information.

What will be your BSA stock in terms of (mg/ml)?

What will be the final volume of your working solution?

You must use 1:10 dilution of the BSA stock solution to give you 10%.

For instance, if you want to prepare 1mg/ml BSA stock, then you weigh 10mg BSA powder in 10ml milli Q/distilled water. While dissolving BSA, gently swirl the tube till the BSA dissolves completely.

From the stock, if you need 10% say in 10ml, then you use the formula

C1V1=C2V2

C1=100% BSA stock (1mg/ml)

V1= volume required

C2= 10% BSA solution

V2= 10ml

Therefore, 100 x V1= 10 x 10

V1 = 1ml

So, you will take 1ml of 1mg/ml BSA stock and make up the volume to 10ml with milliQ/distilled water to give you 10% diluted BSA solution (0.1mg/ml).

Best.

Hello Malcolm Nobre thank you a lot for the explanation

For a 10% BSA-solution, dissolve 10 g in, say, 50 mL of solvent, and then make the solution to 100 mL in a volumetric flask. Dissolving BSA is difficult, as it becomes gluey upon addition of water, you need to vigorously stir for a couple of hours or o/n (but avoid foam!).

The solvent you use depends on what you want to do with the solution later. If your experiment requires PBS, then use that for dissolving. I generally avoid dissolving proteins in pure water, a 10 mM HEPES or Tris buffer is a good alternative if you need the solution for several purposes.

Hi Afaf Hadjer Kherief,

You may use the web tool to calculate

https://www.alfa-chemistry.com/solution-dilution-calculator.htm