how to prepare a BSA stock solution, then how to dilute it to 10%? and sould i dilute it in PBS or water ?
Hello Afaf Hadjer Kherief
You need to provide more information.
What will be your BSA stock in terms of (mg/ml)?
What will be the final volume of your working solution?
You must use 1:10 dilution of the BSA stock solution to give you 10%.
For instance, if you want to prepare 1mg/ml BSA stock, then you weigh 10mg BSA powder in 10ml milli Q/distilled water. While dissolving BSA, gently swirl the tube till the BSA dissolves completely.
From the stock, if you need 10% say in 10ml, then you use the formula
C1V1=C2V2
C1=100% BSA stock (1mg/ml)
V1= volume required
C2= 10% BSA solution
V2= 10ml
Therefore, 100 x V1= 10 x 10
V1 = 1ml
So, you will take 1ml of 1mg/ml BSA stock and make up the volume to 10ml with milliQ/distilled water to give you 10% diluted BSA solution (0.1mg/ml).
Best.
For a 10% BSA-solution, dissolve 10 g in, say, 50 mL of solvent, and then make the solution to 100 mL in a volumetric flask. Dissolving BSA is difficult, as it becomes gluey upon addition of water, you need to vigorously stir for a couple of hours or o/n (but avoid foam!).
The solvent you use depends on what you want to do with the solution later. If your experiment requires PBS, then use that for dissolving. I generally avoid dissolving proteins in pure water, a 10 mM HEPES or Tris buffer is a good alternative if you need the solution for several purposes.
Hi Afaf Hadjer Kherief,
You may use the web tool to calculate
https://www.alfa-chemistry.com/solution-dilution-calculator.htm
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Materials
- Polymers