Hi, everyone.
E8pro::pcambia-1300-CDS-YFP has been transformed into tomato (AC). Although the mRNA of YFP can be detected for first positive screening, but we want to detect if the CDS is expressed in tomato fruit sepecifically in T1 genereation. QPCR is a good way to attain the aim, but is there alternative ways for a quick identification, such as western (yfp antibody) or fluorescence method ?
Thanks in advance.
Ke