Hi everyone,
I have extracted RNA from whole blood store in RNAlater for some years now. I have been using the RiboPure blood kit and adding two DNase treatments to get rid of gDNA. The kit elutes the RNA in 100ul suggesting not going with less than that and my yields are really low (average 10ng/ul).
Any good easy protocol for concentrating RNA that doesn't degrade it too much or reduce yields even more? I am really new in this topics, lab work. So, as more descriptive the better!
Thanks!!!
just take care of time since RNA are unstable and will be degraded with time and temperature.
fred
A SpeedVac is certainly an option if you have access to such a machine. However, be aware that you will also concentrate any contaminating salts!
Much easier:
Article RNA Purification - Precipitation Methods
This is pretty basic molecular biology and a reasonable level of pipetting skill should see you not loosing too much (I used to do this with radioactively labelled RNA!). And you can choose the volume you use to resuspend.
To improve pipetting:
https://bitesizebio.com/biotix-guide-pipetting/ or simply google...
Frida Gorreja did you lost a considerable amount of RNA using that method?
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