Hello everyone,
I am new to In-silico study and trying to make a genome scale metabolic model. I used Cobrapy tool and according to previous researches I used the nearest spp. biomass equation in my model. I am getting very high objective function i.e. in 60 rather than under 1.
Can someone guide me what steps I should take next to optimize the GEM? If there are any useful tools then kindly mention them so I could look into it.
Thank you.
Hi Shakra,
I suggest you look up the below links.
https://www.nature.com/articles/nbt.1614
https://biosustain.github.io/cell-factory-design-course
https://cobrapy.readthedocs.io/en/latest/
Especially, suppose you are new to FBA. In that case, I highly recommend you to read the supplementary information of the What is Flux Balance Analysis paper, which I sent you the link above.
Best,
Hossein
