Please recommend hygromycin kill curve protocol in T47 D cells
My SR785 Dynamic Signal Analyzer keeps rebooting after showing a "WaitFlag Error" in between measurements. I have been unable to find any mention of this error in the service/user manuals...
23 December 2020 8,427 2 View
I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.
06 December 2020 8,479 2 View
I have tried using PowerSoil Pro kit from Qiagen with slight modifications- incubating at 65 degree for 10 mins after adding solution CD1, increasing the number of washes with CD5 to 3 times and...
27 October 2020 7,782 3 View
I have a study which reports PTSD diagnostic status at both 3 and 6 months. In my meta-analysis of the intervention, both 3 and 6-month assessments fall within the same outcome assessment (for...
26 March 2020 2,432 3 View
This is the Base regression: Y = b0*X1+b1*X2+error term I am using an indicator variable Z which takes value 1 for years 4 to 6 and value 0 for years 1 to 3. I am trying to analyse the impact of...
05 March 2020 9,865 3 View
Hello. Our incubator for primary cultures is broken but I need to isolate some islets for both qPCR and estimating the insulin content. Has anyone performed either of these techniques on freshly...
18 November 2019 1,352 3 View
Protein on which i am working is a disordered protein and no crystal structure of this is available on PDB. I have modelled structure using I-TASSER. Out of top five models generated by I-TASSER I...
11 August 2019 9,604 5 View
Hi, In my meta-analysis, the intervention is often compared to more than 1 control group. I am analysing a dichotomous variable (PTSD diagnosis). With continuous outcomes (such as symptom...
30 April 2019 8,168 2 View
I'm having manumaster comeercial microwave oven with 1.1kW power and 2.45 GHz Frequency. I want to convert it into sintering furnace and for the same i have managed tooling requirements like...
25 April 2019 8,401 3 View
Or it can be done only after drying the hydrogels (gravimetric method)
24 October 2018 5,870 4 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
10 December 2020 5,548 3 View
Hi, I am Zaizhi. I would like to know where I can find a database of antibiotics-dose required to kill different bacteria. I found a few years ago. There, I was able to know the range of dose of...
08 November 2020 9,276 3 View
how can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??
28 October 2020 2,675 8 View
Hi, I'm struggling quite a bit, Hope someone can help. My research question is roughly to what extent (and what) characteristics of murder and manslaughter cases are related. The data consists...
05 October 2020 3,958 8 View
I am currently doing an experiment in my faculty lab and I need living micro-algae to be transported from other countries. The samples will be done in Morocco and Spain and they will be sent to...
08 September 2020 4,424 2 View
Does the pain really goes away or it is something else ??
08 September 2020 133 19 View
How do people usually measure bacterial interations? Thank you!
20 August 2020 7,472 3 View
As hydrogel was prepared in acidic medium, released acid from hydrogel kills cells in in-vitro test. I am wondering to compromise such issue.
12 August 2020 9,319 3 View
Hi there, I'm trying to build a killing curve for HEK-293T towards hygromycin B for the following transfection experiment. I plated the cell at 1.3X10^5 cell/well in the 24 well plates the night...
30 June 2020 7,368 1 View
