Please recommend hygromycin kill curve protocol in T47 D cells
can we dope any metal (Cu, Zn, Mg) on silver or gold nanoparticles and how can it be done ?
06 May 2024 5,953 3 View
I am working on tobacco intervention and developing communication strategy, for that a questionnaire was developed, population is Indian tobacco users.
13 April 2024 9,324 2 View
I made two electrode devices for the supercapacitor. I combined activated carbon and PVDF for negative electrodes in an 80:10:10 ratio. I combined active working material, PVDF, and activated...
12 February 2024 2,964 3 View
I want to analyse XPS spectra from xpspeak41. I try every file format such as ASC, .dat but it always shows an error reading file. what should I do? please see the images I attached below
11 February 2024 6,860 3 View
I have 50 ug of Human Her2 / ErbB2 Protein, Tag Free antigen in white powder form and need to make various concentrations of that antigen, such as 50 unit/ml, 10 unit/ml, 0.01 unit/ml, and 0.1...
05 February 2024 9,048 6 View
My work involves the production of melatonin using saccharomyces cerevisiae and its quantification. I have tried various fluorescence spectroscopic methods but couldn't find an optimized protocol...
15 January 2024 9,410 3 View
For validation studies, I want to use RT-PCR for genes derived from sequencing data. For that I have to multiplex the reaction to see the expression for more than one gene. How many primers can be...
18 December 2023 8,387 1 View
I am currently working on yeast cells for melatonin production which requires me to quantify melatonin easily and economically. I was hoping that anyone could provide me with an optimized protocol.
09 December 2023 7,037 1 View
I recently did a single cell sort with MCF-7 cells to create my CRISPR cell line. I sorted single cells in a 96-well plate in MEM media with 10% FBS, 10ug/mL insulin, and 1mM sodium pyruvate. It...
28 October 2023 6,320 1 View
Which value is more accurate and why?
17 October 2023 1,418 0 View
"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...
29 July 2024 2,181 7 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
The question us regarding catalase activity check of bacterial strains. I saw an article in which it was written that we can use 30%hydrogen peroxide for catalase activity but in our laboratories...
12 July 2024 8,794 2 View
So were we wrong about acid rain in the 1980s? Can we be sure that the new story: Climate Change, Desiccated Soil, and Monocultures, is correct? Another alternative from the 1980s is the massive...
10 July 2024 4,667 7 View
I read recently that an AI committed suicide. How is it possible for AI systems to kill itself?
07 July 2024 2,678 1 View
I know steam has become a potential for killing invasive plants. I am curious to know whether or not it has been used successfully in killing the plant and the seeds of Mile-a-minute (Persicaria...
19 June 2024 6,216 0 View
The mankind is on the edge of resistance to peace. If you view news you will find that every day there are portions of the world where wars are happening and many innocent people are being killed....
26 May 2024 4,633 3 View
"Testing earthquake prediction algorithms Article December 2021 Vladimir Kosobokov Solovyov Alexander Alekseevich" You all know very well that Short-term earthquake forecast is not a problem. A...
08 April 2024 7,479 0 View
How can I calculate the used dose of substance in vivo (intratesticular) after determining the effective dose of it in vitro (on semen sample)? Say, if 3 mg of a substance is effective to kill...
05 March 2024 1,699 1 View
Why Indians are so distinguished in physicists and mathematicians? The answer to this question has many similarities with a previous article: Why do Russians excel so much in mathematics and...
15 February 2024 9,294 1 View
