I am planning to perform a colony count using the serial dilution methods on wound swabs transported in Amies transport medium. Could anybody please recommend a protocol to the above?
Hello. Can someone please explain the chemistry behind the assay in the following paper? Thank you RW https://www.sciencedirect.com/science/article/abs/pii/0003269789900043?via%3Dihub
27 September 2023 6,160 0 View
Hi I'm looking to obtain genomic DNA from several vibrio species (excluding cholrae), for cloning purposes. Any recommendations on where I can get this material (besides ATCC)? Specifically...
21 September 2023 3,316 0 View
Are there any studies done regarding this phenomenon?
01 September 2021 9,383 3 View
My protein is 28kda and is some what hydrophobic with a pI of 6 and has a his-tag. We typically purify in 1x TBS with low salt at pH 7.5. During dialysis and or concentration the protein...
18 September 2020 6,646 11 View
Kite diagramme that i am expecting to draw is population dynamics of animals in a national park Sri Lanka over period from Jan 2018 to Dec 2019.
10 January 2020 1,378 2 View
07 February 2018 3,943 1 View
Hello I have human iPS cells that are being thawed and cultured in mTeSR1 media. I want to transition them to E8 media. In the past we have had very limited success with this type of media...
31 October 2017 1,878 4 View
Please explain how the p-value and SD is calculated using Cp values.
15 July 2014 7,937 3 View
01 January 1970 1,868 0 View
01 January 1970 3,523 0 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
29 July 2024 5,622 3 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
The application of estrogen to wounds reduced inflammatory responses and promoted angiogenesis and woundcontraction more than the two other standard treatment methods.
09 July 2024 8,236 0 View