I want to test 96 genotypes planted on a single tray (96 small pockets, 3 seedlings per pocket) for blast disease resistance. I will spray spore suspension on 2 weeks old seedlings. I want to obtain uniform spraying on each genotype. I know that the general method is to spray until runoff. But is there any other method that can be used to get more precise spraying?
droplet or spray elicit differential responses as in MM of https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825148/ Thus you may select the way you find opportune
1. use surfactant to to ensure complete runoff that prevents droplets remaining at leaf edge.
2. Use runoff and when you think that all leaves are covered, check the bottom side. You will find in many cases that it is not covered until you keep spraying more and more.
3. Try to use a fan with a slight air flow while you are spraying to "mix" the leaves to ensure that the underside is covered.
Thank you Isaac Klein..I know that. But I read somewhere that spraying until runoff is the least uniform method. That is why I was thinking if there is any other method to obtain uniformity or not.
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Hi Anik
You can find useful references on attachment
https://books.google.com/books?isbn=0873716388
7
https://extension.msstate.edu/sites/default/files/publications/publications/p2705_0.pdf
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