20 November 2012 1 2K Report

A lab scale procedure

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Maximum passage for Ewing sarcoma cell lines?

I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities

20 May 2024 596 4 View

Experience on subculturing and feeding RD-ES cell line?

Hi - I am looking for advice on protocol for subculturing and feeding RD-ES line. THey seem to grow semi-adhered and not sure if I should treat them as cells in suspension or adhered. Thank you!

09 May 2024 8,224 1 View

Do you suggest any web based or app that could be used to manage bookings of lab related equipment?

We would like to manage in an efficient way our newly conceived flow cytometry and and would like to find a good way to manage our bookings Please Help

21 April 2024 845 1 View

Is this a good titile?

"Architectural Impressions: Exploring the Lifelong Influence of Childhood Environments on Memory and Identity"

21 March 2024 6,448 1 View

How to adjust the PCR program to amplify large amplicon as 7Kb?

I use a Q5 polymerase to amplify a 7 kb fragment from a genomic DNA but get no results. I use the stated protocol in NEB website. Any suggestions to modify the PCR protocol so I can get the...

19 March 2024 1,945 7 View

How to analyze OpenArray data?

In my experiment, I have 8 experimental groups (n=8 rats); one control and 7 treatments. I have performed an OpenArray for each group, so I have 8 OpenArrays. In each OpenArray I have included 112...

18 October 2023 9,514 0 View

Is it necessary to starve cells while using a dual luciferase assay to detect expression on TGF-b cell signalling?

is it necessary to starve cells while using a dual luciferase assay to detect expression on TGF-b cell signaling? I will perform an experiment and I would like to know the pros and cons of...

01 October 2023 6,463 2 View

Is it possible to have a luciferase signal and a fluorescent protein at the same time in a stable cell line?

Hello Dear all! I would like to know if it is possible to have a stable cell line with a response element attached to a luciferase signal, also, Could it be possible to have in the same stable...

10 September 2023 2,670 2 View

How to induce sporulation in in vitro cultures of Alternaria spp.?

Im working with in vitro cultures of Alternaria, however this doesn´t respond to the variations that I have treated in the media following different methodologies to induce sporulation found in...

08 August 2023 9,238 1 View

Do you know if there are Issues and concerns regarding the use of a plasmid containing a Luciferase and a Fluorescent protein together?

I would like to transfect HEK293 with a plasmid containing a luciferase (downstream of a response element to this pathway) to check a cell signal pathway, but in order to have a population of...

20 July 2023 852 1 View

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How to improve peaks separation in HPLC?

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