i intend to carry an experiment to determine some testicular functional indices
You can use an artificial vagina. What I did was collected sperm from female vagina after coitus. But this method produce variable results and you have to standardize the procedure.
Would you do this after the sacrifice of the animal or keeping it alive? Any way, you can have spz by chirurgical way at the epididymal level.
Dear Dr. Nwonuma Charles
I hope these articles can help you in collecting sperms from rats.
http://www.jove.com/video/184/harvesting-sperm-and-artificial-insemination-of-mice
Best Regards
Dear Dr. Charles,
You can use the epididymal or vas deferens tissues to obtain sperm from rats. Alternatively, electroejaculator with probe (7mm diameter) can successfully be used for semen collection undder anaesthesia.
Best wishes,
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