How to obtain more and pure nanoparticles from colloid solution?

12 December 2018 0 4K Report

Nanoparticle colloid solution has been synthesized by lots of researchers, however, there were few efficient approach to enhance the quantity and purity. Please tell me some suggestions, thank you.

Badges
Science topic

More Weidong Huang's questions See All

What's the spectra look like for FRET?

A single fluorescent spot (donor and acceptor were merged as one spot) was obtained as 300 frames through two-channel equipment, the fluorescent intensity of which was analyzed separately. Then I...

03 April 2014 4,307 2 View

Is a laser power appropriate for single molecule imaging?

The laser power is about 0.2 mW above the coverglass, is that able to obtain clear single molecule fluorescence spots?

03 April 2014 3,164 2 View

What is the hybridization time between two complementary oligonucleotides?

I have two complementary oligonucleotides (about 25 nt). How long will they hybridize into dSDNA in a certain buffer at room temperature? Are there some differences between immobilization and...

02 March 2014 1,409 2 View

Does anyone know how to eliminate fluorescence impurities on the coverslip?

There were some fluorescence impurities on the coverslip when it was immobilized on the microscope without adding any sample that confused with the real fluorescence spots. The coverslip was...

02 March 2014 9,667 12 View

How can I identify a single molecule using fluorescent microscopy?

During the process of observing fluorescence, how do I distinguish the real sample from impurity? In addition, how can I identify whether the fluorescence belongs to single molecule or not?

02 March 2014 2,819 3 View

Can someone advise on the use of the laser power during detecting fluorescence molecules?

Now I want to detect fluorescence molecules excited by laser, the power of which is 0.5 mW detected by power meter above the sample. So I want to know whether it is enough to obtain clear images?

02 March 2014 1,001 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

What are the modern methods for detecting nanoparticles?

Some of the techniques I am familiar with are standard. I would like to know which of the two methods under study is preferable, as well as the cost and list of particles and devices needed in...

22 July 2024 1,921 3 View

When making abraxane with Nab tech, why is 9:1 Chloroform&EtOH used as solvents?

Is it the best optimization ratio based on experiments?

22 July 2024 8,170 0 View

The measurement of cellular uptake of nanoparticles?

What protocol can be used to measure the cellular uptake of a nanoparticle? Is there a way to get more of a non-targeted nanoparticle into the cell? Why a fluorescent nanoparticle does not enter...

18 July 2024 7,153 2 View